Funding for rural businesses would generate more than 6,000 new jobs and support the growth of rural enterprises, it said.
The latest round of Rural Development Programme funding was formally announced by Lord Gardiner on Saturday (29 July).
For the first time under the current scheme, specific funding will be available to support new rural broadband projects.
It will also provide significant amounts of funding to on-farm businesses to invest in new infrastructure such as new buildings and machinery.
The current Rural Development Programme for England is expected to generate 6,750 new jobs.
Already more than 1,400 projects have been agreed which are expected to create over 2,300 jobs.
The grants will also fund landowners to improve farm productivity and invest in rural tourism opportunities.
Rural Affairs Minister Lord Gardiner said: "One in three businesses in this country are based in the countryside, and this government is committed to providing the support they need to create a strong and prosperous rural economy.
"This funding will make sure businesses in remote locations can get online, help farmers install cutting-edge technology, create new tourist hotspots and bring high quality jobs to rural communities across the country."
This round of funding will include: £30m to improve rural broadband.
Broadband grants will encourage growth by helping provide broadband services at speeds of 30Mbps or faster where this is not available or planned.
They will supplement existing Department for Digital, Culture, Media and Sport investment in rural broadband.
Some £45m will help rural businesses grow and invest in new equipment.
Rural businesses, including those engaged in tourism and food production, can apply for funding to invest in their company, helping them to expand, diversify, and invest in new technology.
In addition, £120m is earmarked for projects that improve farm productivity.
This money will help farmers, foresters and landowners manage their land more effectively.
Funding will be available for a wide range of purposes, including woodland management equipment, creating on-farm reservoirs and using water more efficiently.
Lord Gardiner made the announcement during a visit to the Suffolk Cookhouse project in Ipswich, which received £422,000 in 2012 to open a new food hall. The hall is now one of the biggest tourism hubs in the region, bringing together local artists and food producers, and creating 45 jobs.
The first grant funding that will become available will be for reservoirs and forestry equipment and these are expected to be open for applications from Monday (31 July).
Funding for broadband, rural business support, on-farm food processing, arable and horticultural productivity and resource efficiency will be made available later in the year.
A further £6.6m will be available for animal health and welfare projects.
This funding is part of planned investment of at least £3.5 billion into our rural economies by 2020 under the Rural Development Programme for England.
This will support the third of businesses in the UK which are based in the countryside.
Defra said all projects agreed before the UK leaves the European Union will be guaranteed for their lifetime.
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