The partnership aims to solve the problem of illegal "off-road" activity on fells and agricultural land, especially in the southern area of the national park.
Not only is illegal off-roading inconsiderate and damaging the environment, 4x4 drivers and motorcyclists could also see vehicles seized by police.
Inspector Kevin Spedding of Cumbria Constabulary said there was also an unacceptable impact on the quality of life of rural residents and tourists.
"Cumbria Constabulary is committed to working with local communities and partner agencies in combating the minority of irresponsible riders and drivers who indulge in illegal off-road activity."
Other partners in the initiative include the Lake District National Park Authority, Natural England, the Forestry Commission, parish councils and local community representatives.
"We know there will be no quick fixes, but there is a determination across all parties to combat this issue in any way they can," said Inspector Spedding.
The partnership was formed in response to the local community's concern and frustration that this issue was not being tackled effectively.
A spokesman for the Campaign Against Rogue Motorcycling said local people could make a vital contribution towards tackling rogue motorcycling.
"We are appealing to all walkers to observe, record and to report to the police any incident of illegal riding of motorbikes."
Illegal off-roading is also of great concern to legal off-roaders who have worked hard to build up good relations with landowners and local communities.
The Cumbria Group of the Trail Riders Fellowship – which represents those who ride road legal motorcycles on legal highways – said it supported the initiative.
"We are very upset that a small minority of 'bandits' sully our image and cause such aggravation."
New warning signs will now be posted on a number of locations reminding those involved that their activity is illegal.
Anyone with any information in relation to off-roading offences being committed should call 0845 3300247.
